IADR Abstract Archives
A Comparison of Different Bone Graft Substitutes With Apatite Structure in Vivo
Objectives: The most obvious alternative to autogenous bone graft has been allograft bone, which has been widely used in the U.S. Although the risk of transmission of disease is much lower than with blood and blood products, it has occurred. Therefore, many types of bone graft alternatives that have no risk of infection have been developed, but there is no substitute for bone graft yet available which embodies all the qualities of autogenous bone. We have developed a novel bone graft substitutes “Cytrans Granules” focusing on synthetic carbonate apatite(CO3Ap), which is the main component of human bone. In this study, we aimed to evaluate the efficacy of CO3Ap in peri-implant bone defect model in dog.
Methods: Animal experiment was performed at Hamri Co., Ltd., which was approved by the Institutional Animal Care and Use Committee (IACUC） of Hamri Co., Ltd.; IACUC approval number-No. 15-H066. Freeze-dried bone allograft (FDBA) as an allogeneic bone, deproteinized bovine bone mineral (DBBM) and Hydroxy apatite (HAp) were used as control. 2 standardized 7×2×6 mm box-shaped defects were bilaterally created, and φ2.2 mm dental implants were placed in distal of the defects. Each sample were filled in the bone defects (n=3). After healing period of 4 weeks, the samples along with the surrounding tissue were carefully harvested from the mandibular bone. Subsequently, Villanueva Goldner stain was applied to all sections and observed using a light microscope.
Results: Histological evaluation revealed a relatively higher augmented area for CO3Ap groups compared to others. FDBA had the second highest new bone formation, but only up to half the height of the defect. DBBM had the second lowest formation of new bone. HAp had the lowest formation of new bone.
Conclusions: From the result of this study, CO3Ap showed the same or better bone regeneration ability as HAp, DBBM and FDBA in peri-implant bone defect. It was reported that a high carbonate content in substitute with apatite structure led to increased osteoclastogenesis in culture. The carbonate content is in the order of CO3Ap>FDBA>DBBM>HAp. We think that the higher carbonate content, the osteoclasts are activated and the faster the bone remodeling, resulting in more new bone formation.
Methods: Animal experiment was performed at Hamri Co., Ltd., which was approved by the Institutional Animal Care and Use Committee (IACUC） of Hamri Co., Ltd.; IACUC approval number-No. 15-H066. Freeze-dried bone allograft (FDBA) as an allogeneic bone, deproteinized bovine bone mineral (DBBM) and Hydroxy apatite (HAp) were used as control. 2 standardized 7×2×6 mm box-shaped defects were bilaterally created, and φ2.2 mm dental implants were placed in distal of the defects. Each sample were filled in the bone defects (n=3). After healing period of 4 weeks, the samples along with the surrounding tissue were carefully harvested from the mandibular bone. Subsequently, Villanueva Goldner stain was applied to all sections and observed using a light microscope.
Results: Histological evaluation revealed a relatively higher augmented area for CO3Ap groups compared to others. FDBA had the second highest new bone formation, but only up to half the height of the defect. DBBM had the second lowest formation of new bone. HAp had the lowest formation of new bone.
Conclusions: From the result of this study, CO3Ap showed the same or better bone regeneration ability as HAp, DBBM and FDBA in peri-implant bone defect. It was reported that a high carbonate content in substitute with apatite structure led to increased osteoclastogenesis in culture. The carbonate content is in the order of CO3Ap>FDBA>DBBM>HAp. We think that the higher carbonate content, the osteoclasts are activated and the faster the bone remodeling, resulting in more new bone formation.