IADR Abstract Archives

Role of Stem Cells Around Carbonate Apatite as Bone Substitute

Objectives: The use of bone substitutes has become an important component of safe implant treatment. In recent years, bone substitutes containing carbonate apatite (CO3Ap), a major inorganic component of bone, have been developed. However, the detailed mechanism by which CO3Ap works as an effective bone substitute remains unclear. We focused on mesenchymal stem cells (MSCs), which are also strongly involved in wound healing in the oral cavity, and compared the differences between CO3Ap bone substitutes and hydroxyapatite (HAp) and β-tricalcium phosphate (β-TCP) materials in animal and culture experiments.
Methods: In vivo experiment: 6-week-old male Wistar rats had maxillary right first and second molars removed, and CO3Ap, HAp, and β-TCP inserted into the site. MSC accumulation surrounding the extraction site was evaluated. In vitro experiment: MSCs from rats were co-cultured with CO3Ap, HAp, β-TCP or no material (control). Cell morphology, proliferative capacity, differentiation, and expression of some growth factors were evaluated. Additionally, changes in the calcium concentration of the culture solution were measured as an influential factor.
Results: In vivo experiments: Healing of the extraction sockets were significantly promoted in the CO3Ap group, together with strong accumulation of MSCs. In vitro experiment: The differentiation potential and the amount of calcium deposition were significantly lower in the CO3Ap and HAp groups than in the control and β-TCP groups. There was no significant difference in proliferative ability between the bone substitutes. However, an increase in IGF-I and VEGF was confirmed only in the CO3Ap group.
Conclusions: When CO3Ap was used as a bone substitute, MSCs accumulated in the surrounding tissues. However, MSCs seemed not to be directly involved in tissue repair, but were indirectly involved by releasing growth factors such as IGF-I and VEGF from MSCs. These findings suggest that CO3Ap promotes bone formation around the extraction socket and soft tissue healing.

2022 IADR/APR General Session (Virtual)

2022
0334
Stem Cell Biology Research
  • Takahashi, Ryosuke  ( Kyushu University , Fukuoka , Fukuoka , Japan ;  Kyushu University , Fukuoka , Fukuoka , Japan )
  • Atsuta, Ikiru  ( Kyushu University , Fukuoka , Fukuoka , Japan )
  • Narimatsu, Ikue  ( Kyushu University , Fukuoka , Fukuoka , Japan )
  • Zhang, Xiaoxu  ( Kyushu University , Fukuoka , Fukuoka , Japan ;  Kyushu University , Fukuoka , Fukuoka , Japan )
  • Egashira, Yuki  ( Kyushu University , Fukuoka , Fukuoka , Japan ;  Kyushu University , Fukuoka , Fukuoka , Japan )
  • Koyano, Kiyoshi  ( Kyushu University , Fukuoka , Fukuoka , Japan )
  • Ayukawa, Yasunori  ( Kyushu University , Fukuoka , Fukuoka , Japan )
  • JSPS KAKEMHI Grant Number JP 23592888
    I.A. and K.K. belong to the Division of Advanced Dental Devices and Therapeutics, Faculty of Dental Science, Kyushu University. This division and Cytrans are endowed by GC Corporation, Tokyo, Japan. GC Corporation had no specific roles in the presentation
    Interactive Talk Session
    Stem Cell Biology I
    Wednesday, 06/22/2022 , 01:30PM - 03:00PM